Smoke billows from buildings during an operation by Syrian government forces to retake control of Leramun, on the north-west outskirts of Aleppo. Photograph: George Ourfalian/AFP/Getty Images
Supplies are quickly running out and women, children and men will begin to starve unless the armed parties on the ground are made to open the way for humanitarian aid, said Save the Childrens Syria director, Sonya Khush.
The parties responsible for this are in the meantime feeling bolder in committing their violations, she added, warning that bombing raids had targeted civilian infrastructure and supplies.
A food warehouse with almost 10,000 parcels was destroyed, and fuel needed to power medical facilities and water pumps was running dangerously low, the charities warned, and Unicef said medical facilities were also targeted.
Four hospitals in eastern Aleppo city … and a blood bank were reportedly hit several times on 23 and 24 July disrupting key life-saving health, the UN agency said. Health facilities in Syria are being attacked with alarming ferocity.
There were two bombing raids in under 12 hours on the only working paediatric hospital in the city. A two-day-old baby was among the casualties.
Earlier this week, the United Nations called for regular 48-hour ceasefires in the city to allow food and other assistance in, amid rising concern about the plight of civilians.
On Wednesday, the US military announced that it was launching a formal investigation into an airstrike on 19 July in which scores of people are thought to have been killed. The death toll in an attack near the besieged city of Manbij remains disputed but the UK-based monitoring group Airwars has concluded that at least 74 people died.
The UNs top Syria envoy also said this week that it hoped to restart soon intermittent peace talks aimed at ending the five-year war . They collapsed in April, partly because of intensifying violence around Aleppo.
The scale and intensity of the fighting have proved a huge challenge for aid groups, often forced to work through intermediaries in areas that are extremely dangerous to operate in even for experienced organisations
Fears of a looming humanitarian disaster in Aleppo come as the US revealed it had suspended more $200m (150m) of aid projects, providing food, shelter and medical support to Syrians, because of corruption fears.
The Isis truck bomb launched a few hundred miles east of Aleppo, in the strategic border town of Qamishli, was the bloodiest attack yet on a town that has been a frequent target for Isis militants.
Wednesdays explosion was so powerful it shattered windows and caused minor injuries inside Turkey. Video footage showed people fleeing blazing rubble before a second explosion, reportedly from a fuel tank that caught fire, sent them diving for cover.
Qamishli is under joint control of Syrian government forces and Kurdish fighters, and Isis said the truck bomb targeted Kurdish military offices. Kurdish forces are leading the ground campaign against Isis further west, currently focused on a push for the city of Manbij.
Suleiman Youssef, a writer and Qamishli resident, said that he heard the first explosion from a few miles away. He told AP the blasts levelled several buildings to the ground, and that many people were trapped under the rubble.
The predominantly Kurdish, US-backed Syria Democratic Forces has been the main force fighting Isis in northern Syria. It has captured significant territory from the extremists in the past two years.